| TMB One Component HRP Microwell Substrate (SUB1)
is our most popular substrate and provides the reference for performance of all the other HRP substrates offered. SUB1 is ideal for most ELISAs where the target is in the ng-pg range. We recommend starting with this formulation when developing an assay to estimate the level of sensitivity. Then, if necessary, change formulations to increase or decrease sensitivity when optimizing your assay.
Absorbance for TMB = 370 or 620 - 650
nm.
TMB Super Sensitive One Component HRP Microwell Substrate (SUB2)
is our most sensitive TMB buffer. SUB2 is approximately 40-fold more sensitive than our most popular substrate - SUB1. SUB2 is useful when detecting very low levels of a target molecule, with samples that must be highly diluted (1:10,000), to amplify the signal when using antibodies with low binding capacity, with samples that exhibit high steric hindrance, and to shorten the incubation time of the assay.
Absorbance for TMB = 370 or 620 - 650
nm.
TMB Slow Kinetic One Component HRP Microwell Substrate (SUB3)
exhibits roughly 25% less sensitivity than SUB1. Lower sensitivity substrates are ideal for ELISAs where the test samples contain high levels of the target molecule, for assays with long incubation periods (such as overnight incubations), and for assays that simply do not require a high level of sensitivity.
Absorbance for TMB = 370 or 620 - 650
nm. |
|
ABTS One Component HRP Microwell Substrate (SUB4)
Use ABTS for samples with high levels of the target molecule; for samples that do not have to be diluted very far (1:100); for assays with long incubation periods, (such as overnight incubations); and for assays that simply do not require a high level of
sensitivity. Absorbance for ABTS = 405 - 410
nm.
AP One Component pNPP Microwell Substrate (SUB5)
is used in ELISA assays to detect alkaline
phosphatase-conjugated molecules. This formulation of pNPP substrate contains a stabilizer to extend the shelf life of the substrate on your
benchtop. Absorbance for pNPP = 405 - 420 nm.
TMB One Component HRP Membrane Substrate (SUB6) is useful for immunoblotting applications where
HRP-conjugated molecules are used for detection. SUB6 reacts with HRP yielding a dark blue reaction product. The formulation of this substrate does not contain any aprotic solvents.
Absorbance for TMB = 370 or 620 - 650
nm.
BCIP/NBT One Component AP Membrane Substrate (SUB7)
use BCIP(5-bromo-4-Chloro-3’-Indolyphosphate
p-Toluidine Salt) and NBT (Nitro-Blue Tetrazolium Chloride) for immunoblotting applications where alkaline
phosphatase-conjugates molecules are used for detection. NBT serves as the oxidant and BCIP is the AP substrate. SUB7 reacts with AP yielding a bluish-purple reaction product. |
