Stop solutions for HRP substrate AP substrate TMB ABTS BCIP NBT Stop reagent ELISA solutions from ICT ELISA signal generation ELISA detection

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Stop Solutions
Stop the reaction

 

Stop Solutions are used to prevent further color development of the substrate in an ELISA assay. The chromogenic signal is arrested in endpoint assays so that the readout is indicative of the time the assay is stopped. Stop solution may also be added at the final time point in a kinetics assay.

ICT offers 3 reliable stop solutions:

1. Stop Solution for TMB Microwell Substrates (STOP1)
2. Stop Solution for ABTS Microwell Substrate (STOP2)
3. Stop Solution for AP Microwell Substrate (STOP3)

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Stop Solution for TMB Microwell Substrates (STOP1) is an easy-to-use liquid stop solution. STOP1 is suitable for use with our TMB substrates SUB1, SUB2, and SUB3. This TMB stop solution changes the chromogen from blue to yellow, and hence the absorbance from 620 nm to 450 nm. 

Stop Solution for ABTS Microwell Substrate (STOP2) is an easy-to-use liquid stop solution. STOP2 is compatible with our ABTS substrate SUB4. This ABTS stop solution does not change the color or the absorbance of the chromogen, and so the absorbance is read at 405 nm to 410 nm. 

Stop Solution for AP Microwell Substrate (STOP3) is an easy-to-use liquid stop solution. STOP3 is compatible with our alkaline phosphatase substrate SUB5. This AP stop solution does not change the color or the absorbance of the chromogen, and so the absorbance is read at 405 nm to 420 nm. 

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ICT has cool post-it notes - free with every order! With each order...
Designed as a 96-well microtiter plate, these cool 4x6" sticky-notes will help keep your experiments organized.

Help!  I can't get my assay to work!
    
Just give use a call at 1-800-829-3194.  To help you develop your assay, ICT has provided some background information on ELISA technology (see What is an Immunoassay , What's in a test and what is an ELISA protocol? ), some calculation worksheets (see How Do I Coat Plates), and offers consultation services in assay development (see Immunoassay Development).   

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01/05/2010 04:25 PM
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Please contact Sally Hed with questions 952-888-8788 x10 sally@immunochemistry.com .